2012•ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSISRequires access

Relationship between Single Nucleotide Polymorphism of POU1F1 Gene and the Growth Traits of Local Pig Breeds

Limin Wei

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Abstract

Six Chinese local pig breeds were used as experimental examples,a mutation in intron of pig Pit-1 gene was found by using PCR-SSCP analysis.The result of population genetics analyses showed that in the mutation of P2,the frequency of BB genotype was significantly higher than that of AA genotype in Diannan Small ear pig and Xiang pig and there was no polymorphism in Hainan native pigs.In the mutation of P3,the frequency of CD genotype was significantly higher than that of DD genotype and had intermediate polymorphism in the six Chinese local breeds.The least square analysis showed that the different genotypes of intron1 of POUlF1 gene did not affect WZSP body weight of 2 month,4 month,8 month and daily gain.

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What this paper is about

Six Chinese local pig breeds were used as experimental examples,a mutation in intron of pig Pit-1 gene was found by using PCR-SSCP analysis.The result of population genetics analyses showed that in the mutation of P2,the frequency of BB genotype was significantly higher than that of AA genotype in Diannan Small ear pig and Xiang pig and there was no polymorphism in Hainan native pigs.In the mutation of P3,the frequency of CD genotype was significantly higher than that of DD genotype and had intermediate polymorphism in the six Chinese local breeds.The least square analysis showed that the different genotypes of intron1 of POUlF1 gene did not affect WZSP body weight of 2 month,4 month,8 month and daily gain.

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Available abstract

Six Chinese local pig breeds were used as experimental examples,a mutation in intron of pig Pit-1 gene was found by using PCR-SSCP analysis.The result of population genetics analyses showed that in the mutation of P2,the frequency of BB genotype was significantly higher than that of AA genotype in Diannan Small ear pig and Xiang pig and there was no polymorphism in Hainan native pigs.In the mutation of P3,the frequency of CD genotype was significantly higher than that of DD genotype and had intermediate polymorphism in the six Chinese local breeds.The least square analysis showed that the different genotypes of intron1 of POUlF1 gene did not affect WZSP body weight of 2 month,4 month,8 month and daily gain.

Key concepts: Genotype, Biology, Genetics, Single-nucleotide polymorphism, Polymorphism (computer science), Single-strand conformation polymorphism, Gene, Genotype frequency

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