Polymorphism Analysis of POU1F1 Promoter Region and It's Association with Growth Traits

【Objective】 The aim of current study is to obtain the polymorphisms of POU1F1 promoter region,investigate the distribution of these polymorphisms among different pig breeds and detect it's association with growth traits.【Method】 PCR cloning,DNA sequencing and PCR-RFLP techniques were applied to detect the polymorphisms of POU1F1 promoter region,and the general linear model was used to analyze it's association with growth traits.【Result】 Five polymorphism sites were identified in the 1.5 kb POU1F1 promoter region,and the frequency of A allele in the 5 bp insertion or deletion mutation was the highest in introduced pig breeds,moderate in bred breeds,and lowest in Chinese native breeds.The general linear model analysis indicated that this polymorphism site was significantly associated with growth traits.LSD showed that the pigs with AA genotype had higher body highness,body length,circumference and body weight at 6-month-old of age compared with that of AB and BB pigs,but the difference of body highness,circumference and body weight between AB and BB pigs was not significant,while the body length of AB pigs was significantly higher than that of BB pigs.【Conclusion】 The A allele in 5bp insertion or deletion mutation site is favorable,and it could be useful in molecular marker assisted selection for growth traits.