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2015•Swine ProductionRequires access

Analysis of IGFBP6 Gene Polymorphism in Hainan Native Pig Breeds

Sun Ruipin

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Abstract

The PCR-SSCP in IGFBP6 gene and their correlations with growth traits were investigated in Hainan WZS pig in a closed breeding colony, in Hainan black pigs(Lingao pig and Tunchang pig). The results showed there were two alleles and three genotypes in PCR products of IGFBP6 gene.Subsequent sequencing results showed that they were all there was variation in the corresponding area.The result of population genetics analysis,showed that in the mutation of P1, the frequency of BB genotype were significantly higher than AA genotype, respectively.In the mutation of primer P2, the frequency of CC genotype were higher than other genotypes. The least square analysis showed that the body weight of 2, 8 and 12-month-old pigs were higher than other month pigs,but there was no significance(P0.05), respectively.

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What this paper is about

The PCR-SSCP in IGFBP6 gene and their correlations with growth traits were investigated in Hainan WZS pig in a closed breeding colony, in Hainan black pigs(Lingao pig and Tunchang pig). The results showed there were two alleles and three genotypes in PCR products of IGFBP6 gene.Subsequent sequencing results showed that they were all there was variation in the corresponding area.The result of population genetics analysis,showed that in the mutation of P1, the frequency of BB genotype were significantly higher than AA genotype, respectively.In the mutation of primer P2, the frequency of CC genotype were higher than other genotypes. The least square analysis showed that the body weight of 2, 8 and 12-month-old pigs were higher than other month pigs,but there was no significance(P0.05), respectively.

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Available abstract

The PCR-SSCP in IGFBP6 gene and their correlations with growth traits were investigated in Hainan WZS pig in a closed breeding colony, in Hainan black pigs(Lingao pig and Tunchang pig). The results showed there were two alleles and three genotypes in PCR products of IGFBP6 gene.Subsequent sequencing results showed that they were all there was variation in the corresponding area.The result of population genetics analysis,showed that in the mutation of P1, the frequency of BB genotype were significantly higher than AA genotype, respectively.In the mutation of primer P2, the frequency of CC genotype were higher than other genotypes. The least square analysis showed that the body weight of 2, 8 and 12-month-old pigs were higher than other month pigs,but there was no significance(P0.05), respectively.

Key concepts: Genotype, Biology, Genetics, Allele, Polymorphism (computer science), Allele frequency, Donkey, Primer (cosmetics)

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