Extraction of Porphyra haitanensis polysaccharides and its anti-influenza virus activity

Porphyra haitanensis polysaccharide was extracted by four methods, among those that the extracting rate of synthesis method was the highest, reaching 16.58%. As shown by the gas chromatographic and chemical analysis, the monosaccharide of soluble polysaccharide is comprised of galactose, 3, 6-AG, mannose and a little xylose. The crude polysaccharide was purified by DEAE-23 and the neutral polysaccharide was eluted by distilled water and the polyanion polysaccharide gradually by 1.0-2.0?mol/L NaCl. Their contents were 4.12% and 95.88%, respectively. The polyanion polysaccharide was degraded by vitriol and separated by ultrafiltration .The polysaccharide F1(3?500MW30?000) and F2(MW30?000)were obtained, the proportion of 4∶1. The chick embryo experiments showed that the porphyra polysaccharides had antiinfluenza A1 virus activity , and F1F2 neutral polysaccharide.