Metabolic flux analysis of L-ornithine biosynthesis by Corynebacterium glutamicum

The metabolic flux balance model of L-ornithine synthesis by Corynebacterium glutamicum 1009 was constructed.Using the model,the metabolic flux distribution was determined and the optimal flux distribution was calculated by Matlab method.Results showed that 58.6% metabolic flux entered the Embden-Meyerhof-Parnas pathway and 41.4% entered the hexose monophophate pathway.Compared with the optimal flux distributions,the production of L-ornithine achieved 65.4% of the theory maximum yield.The production of L-ornithine could be improved from the genetic manipulation and the fermentation control through reducing byproduct of amino acid and organic acid and decreasing the flux of trichloroacetic acid cycle.