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2003•Wuxi Qinggong Daxue xuebaoRequires access

The Metabolic Flux Analysis of Corynebacterium Glutamicum NS611

Cao Zhu-an

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Abstract

In this paper, a metabolic network of the Corynebacterium glutamicum NS611 was estab-lished and modified. It showed that glucose was mainly metabolized through the EMP pathway. In the production phase, the metabolic flux of the pyruvate carboxylation was large. NADPH was not the limiting factor of the metabolism of Corynebacterium glutamicum NS611. The metabolic fluxes of the Corynebacterium glutamicum NS611 with and without nitrogen starvation were compared. It showed that the oxidation of the a-ketoglutarate should be properly retained to ensure the energy supply.

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What this paper is about

In this paper, a metabolic network of the Corynebacterium glutamicum NS611 was estab-lished and modified. It showed that glucose was mainly metabolized through the EMP pathway. In the production phase, the metabolic flux of the pyruvate carboxylation was large. NADPH was not the limiting factor of the metabolism of Corynebacterium glutamicum NS611. The metabolic fluxes of the Corynebacterium glutamicum NS611 with and without nitrogen starvation were compared. It showed that the oxidation of the a-ketoglutarate should be properly retained to ensure the energy supply.

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Available abstract

In this paper, a metabolic network of the Corynebacterium glutamicum NS611 was estab-lished and modified. It showed that glucose was mainly metabolized through the EMP pathway. In the production phase, the metabolic flux of the pyruvate carboxylation was large. NADPH was not the limiting factor of the metabolism of Corynebacterium glutamicum NS611. The metabolic fluxes of the Corynebacterium glutamicum NS611 with and without nitrogen starvation were compared. It showed that the oxidation of the a-ketoglutarate should be properly retained to ensure the energy supply.

Key concepts: Corynebacterium glutamicum, Flux (metallurgy), Metabolic flux analysis, Metabolic pathway, Biochemistry, Corynebacterium, Metabolism, Metabolic network

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