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2006•Journal of Anhui Traditional Chinese Medical CollegeRequires access

Study on Quality Control Methods of Danyutong Granules

Wenjia Zhang

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Abstract

Objective: To establish the quality control methods for Danyutong granules. Method: Herba artemisiae scopariae, Radix bupleuri, Radix scutellariae and Radix et rhizoma rhei in Danyutong granules were identified by TLC; the content of Baicalin was determined by HPLC. Result: The thin layer chromatogram spots were clear without any interference in the blank test;The linearity of Baicalin was in the range of 0.2~1.0μg (r=0.999 9) and the average recovery was 99.6%, RSD=0.99%(n=5). Conlusion: The qualitative and quantitative methods are highly special, sensitive and repeatable, and they can be used as the quality control of Danyutong granules.

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What this paper is about

Objective: To establish the quality control methods for Danyutong granules. Method: Herba artemisiae scopariae, Radix bupleuri, Radix scutellariae and Radix et rhizoma rhei in Danyutong granules were identified by TLC; the content of Baicalin was determined by HPLC. Result: The thin layer chromatogram spots were clear without any interference in the blank test;The linearity of Baicalin was in the range of 0.2~1.0μg (r=0.999 9) and the average recovery was 99.6%, RSD=0.99%(n=5). Conlusion: The qualitative and quantitative methods are highly special, sensitive and repeatable, and they can be used as the quality control of Danyutong granules.

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Available abstract

Objective: To establish the quality control methods for Danyutong granules. Method: Herba artemisiae scopariae, Radix bupleuri, Radix scutellariae and Radix et rhizoma rhei in Danyutong granules were identified by TLC; the content of Baicalin was determined by HPLC. Result: The thin layer chromatogram spots were clear without any interference in the blank test;The linearity of Baicalin was in the range of 0.2~1.0μg (r=0.999 9) and the average recovery was 99.6%, RSD=0.99%(n=5). Conlusion: The qualitative and quantitative methods are highly special, sensitive and repeatable, and they can be used as the quality control of Danyutong granules.

Key concepts: Baicalin, Chromatography, Radix (gastropod), Chemistry, High-performance liquid chromatography, Traditional medicine, Medicine, Biology

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