ScholarLens Back to search
2008•Journal of Northeast Normal UniversityRequires access

RP-HPLC simultaneous determination of Panax notoginseng saponins and ginsenoside in Sanqiangong capsules

Weiyu Zhang

Open publisher page 0 citations

Abstract

To establish simple and reliable RP-HPLC method for quantitative determination of Panax notoginseng saponins and ginsenoside in Sanqiangong capsules at the same time.C18 column was used.The mobile phase consisted of acetonitrile0.2% phosphoric acid(0～30 min,V乙腈∶V磷酸=20∶80;31～80 min,V乙腈∶V磷酸=30∶70).The flow rate was 1.0 mL/min.The UV detection wavelength was 203 nm.The calibration curve of notoginsenoside R1 was linear in the range of 0.42 to 2.10 μg(r=0.999 6)and the average recoveries(n=5)were 96.4%.The calibration curve of ginsenoside Rg1 curve was linear in the range of 1.59 to 10.6 μg(r=0.999 9)and the average recoveries(n=5)were 97.7%.The calibration curve of ginsenoside Rb1 was linear in the range of 1.497 to 9.98 μg(r=0.999 7)and the average recoveries(n=5)were 98.1%.

About this research paper

What this paper is about

To establish simple and reliable RP-HPLC method for quantitative determination of Panax notoginseng saponins and ginsenoside in Sanqiangong capsules at the same time.C18 column was used.The mobile phase consisted of acetonitrile0.2% phosphoric acid(0～30 min,V乙腈∶V磷酸=20∶80;31～80 min,V乙腈∶V磷酸=30∶70).The flow rate was 1.0 mL/min.The UV detection wavelength was 203 nm.The calibration curve of notoginsenoside R1 was linear in the range of 0.42 to 2.10 μg(r=0.999 6)and the average recoveries(n=5)were 96.4%.The calibration curve of ginsenoside Rg1 curve was linear in the range of 1.59 to 10.6 μg(r=0.999 9)and the average recoveries(n=5)were 97.7%.The calibration curve of ginsenoside Rb1 was linear in the range of 1.497 to 9.98 μg(r=0.999 7)and the average recoveries(n=5)were 98.1%.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

To establish simple and reliable RP-HPLC method for quantitative determination of Panax notoginseng saponins and ginsenoside in Sanqiangong capsules at the same time.C18 column was used.The mobile phase consisted of acetonitrile0.2% phosphoric acid(0～30 min,V乙腈∶V磷酸=20∶80;31～80 min,V乙腈∶V磷酸=30∶70).The flow rate was 1.0 mL/min.The UV detection wavelength was 203 nm.The calibration curve of notoginsenoside R1 was linear in the range of 0.42 to 2.10 μg(r=0.999 6)and the average recoveries(n=5)were 96.4%.The calibration curve of ginsenoside Rg1 curve was linear in the range of 1.59 to 10.6 μg(r=0.999 9)and the average recoveries(n=5)were 97.7%.The calibration curve of ginsenoside Rb1 was linear in the range of 1.497 to 9.98 μg(r=0.999 7)and the average recoveries(n=5)were 98.1%.

Key concepts: Panax notoginseng, Calibration curve, Chromatography, Ginsenoside, Phosphoric acid, Chemistry, Ginsenoside Rg1, High-performance liquid chromatography

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
RP-HPLC simultaneous determination of Panax notoginseng saponins and ginsenoside in Sanqiangong capsules — Research Paper | ScholarLens