Determination of ginsenoside Rg_1 and ginsenoside Rb_1 in compound preparation containing Panax notoginseng by RP-HPLC

OBJECTIVE To develop a method for determination of ginsenoside Rg1 and ginsenoside Rb1 in compound preparation containing Panax notoginseng.METHODS An RP-HPLC method was adopted.The analysis was carried out on an analytical column C18(250 mm×4.6 mm,5 μm).The flow rate was 1.0 ml·min-1 and the detective wavelength was set at 203 nm with column temperature of 30℃.The mobile phase consisted of acetonitrile-water(gradient elution).RESULTS The linear range was 0.2124-2.1240 μg(r=0.9999) and the average recovery was 100.24% with the RSD of 1.14%(n=9)for ginsenoside Rg1.The linear range was 0.2108-2.1080 μg(r=0.9999) and the average recovery was 99.71% with the RSD of 0.79%(n=9)for ginsenoside Rb1.CONCLUSION The method is simple,reproducible and suitable for determination of ginsenoside Rg1 and ginsenoside Rb1 in compound preparation containing Panax notoginseng.