Optimization of Cultivation Conditions for Biomass by Spirulina. platensis Using RSM

In this paper, a novel air-lift photo-bioreactor was designed to cultivate the Spirulina platensis, employing the response surface methodology to optimize the culture conditions of biomass concentration. Four critical factors selected for investigation were light intensity, air flow,time of cultivation and volume of medium. Then, research the optimization concentration levels and the relationship between these factors and building a quadratic regression equation with DW as the dependent. According to the experiment results, the ordinal relation from strong to inferior influence the growth of SP is light intensity, time of cultivation, volume of medium, air flow. Finally, by solving the inverse matrix from the equation, the optimal conditions for obtaining the maximum 1.277 g/L of DW are 4400lx, 212.2 L/h, 8.8 d and 7.2 L respectively.