Molecular analysis and database establishment of Vibrio parahaemolyticus by pulsed field gel electrophoresis in Xiamen

Objective To establish pulsed field gel electrophoresis(PFGE)database of Vibrio parahaemolyticus and to analyse the homology during the strains from different sources. Methods The DNA samples of 203 Vibrio parahaemolyticus strains were digested with SfiⅠ and then were analyzed by PFGE and bionumerics software. Results 203 strains were divided into 149 distinctive PFGE patterns 91.13%(185 strains) of strains owned unique PFGE pattern,and a few of strains owned more strains.79 strains from surveillance were divided into 74 PFGE patterns,which indicated that there was no relationship between the typing results and aquatic product,sampling time.124 strains from food poisoning were divided into 76 PFGE patterns.The D value was 99.81% calculated by bionumerics software. Conclusion The PFGE database of Vibrio parahaemolyticus in Xiamen is established,which providing scientific basis for surveillance and emergency response of Vibrio parahaemolyticus in the city.