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2007•Chinese Journal of Public HealthRequires access

Analysis on PFGE molecular subtyping of vibrio parahaemolyticus strains

Shi Xiao-lu

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Abstract

Objective To determine relationship among vibrio parahaemolyticus strains and develop DNA fingerprint database of vibrio parahaemolyticus strains in Shenzhen.Methods Chromosomal DNAs from 56 isolates in agarose were digested with the restriction enzyme NotI,and then were analyzed by pulsed-field gel electrophoresis.PFGE patterns were clustered using Bionumerics software.Results There were 34 distractive PFGE patterns.28 stains belonged to the same cluster.Conclusion There were closely related pandemic clones of vibrio pamhaemolyticus in Shenzhen.The development of DNA fingerprint database would contribute to develop molecular subtyping surveillance network,laboratory-based active surveillance and source tracking for vibrio parahaemolticus.

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What this paper is about

Objective To determine relationship among vibrio parahaemolyticus strains and develop DNA fingerprint database of vibrio parahaemolyticus strains in Shenzhen.Methods Chromosomal DNAs from 56 isolates in agarose were digested with the restriction enzyme NotI,and then were analyzed by pulsed-field gel electrophoresis.PFGE patterns were clustered using Bionumerics software.Results There were 34 distractive PFGE patterns.28 stains belonged to the same cluster.Conclusion There were closely related pandemic clones of vibrio pamhaemolyticus in Shenzhen.The development of DNA fingerprint database would contribute to develop molecular subtyping surveillance network,laboratory-based active surveillance and source tracking for vibrio parahaemolticus.

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Available abstract

Objective To determine relationship among vibrio parahaemolyticus strains and develop DNA fingerprint database of vibrio parahaemolyticus strains in Shenzhen.Methods Chromosomal DNAs from 56 isolates in agarose were digested with the restriction enzyme NotI,and then were analyzed by pulsed-field gel electrophoresis.PFGE patterns were clustered using Bionumerics software.Results There were 34 distractive PFGE patterns.28 stains belonged to the same cluster.Conclusion There were closely related pandemic clones of vibrio pamhaemolyticus in Shenzhen.The development of DNA fingerprint database would contribute to develop molecular subtyping surveillance network,laboratory-based active surveillance and source tracking for vibrio parahaemolticus.

Key concepts: Pulsed-field gel electrophoresis, Subtyping, Vibrio parahaemolyticus, DNA profiling, Biology, Microbiology, Vibrio, Genetics

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