Influence of BuShen JieDu HuoXue Recipe on the blood-producing function in mice of myelosuppression

Objective To investigate the effects of Bushen Jiedu Huoxue Recipe on the blood-producing function in mice of myelosuppression induced by cyclophosphamide.Methods 60 mice were randomly divided into 3 groups,20 in each group:the blank control,the model control and the Bushen Jiedu Huoxue group,the latter two groups of mice were injected cyclophosphamide intraperitoneally for 3 consecutive days at 100 mg/kg daily.The next day after the model was established,the Bushen Jiedu Huoxue group of mice were administrated by gavage with Bushen Jiedu Huoxue decoction for 10 days at 0.36 g/(kg·d),the same volume of physiological saline was given to the other two groups respectively.Thereafter,the peripheral blood was detected on 0、1、3、5、7、10、14 days after model established ;BMNC、hematopoietic progenitor cell colony forming were detected on the 11th day.Result The WBC of the model group and the Bushen Jiedu Huoxue group descended to the lowest point after 1d the model was established,which had obvious significance compared to the blank control group(P0.01),WBC of the model group and Bushen Jiedu Huoxue group both upswung on the 3rd day,while the Bushen Jiedu Huoxue group put back on the 5th day and kept steady,the model group showed up an abnormal raise on the 7th day and then returned to normal.The PLT of the model group descended to the lowest point after 5days the model was established and upswung to normal on the 10th day,while the BushenJieduHuoxue group upswung from the 3rd day and recovered to normal on the 7th day.The lowest point of PLT of the mice in Bushen Jiedu Huoxue group was higher than that of the model group(P0.01).The BMNC、CFU-GM、CFU-E and BFU-E of Bushen Jiedu Huoxue group was higher than the model group and lower than the control group.(P0.01).Conclusion Bushen Jiedu Huoxue Recipe may play a key role in increasing the number of hemopoietic progenitor cell of marrow-depressed mice induced by cyclophosphamide and enhanced the steady recovery of peripheral blood of WBC and PLT.