A Comparative Study of Bushen Jiedu Huoxue Recipe and Yiqi Buxue Recipe on the Prevention of Bone Marrow Suppression Induced by Cyclophosphamide

Objective:To investigate the effect and mechanism of Bushen Jiedu Huoxue Recipe and Yiqi Buxue Recipe on preventing bone marrow suppression induced by cyclophosphamide(CTX) in mice,and provide the basis for clinical compatibility.Method: Eighty Clean grade Kunming mice were used and randomly divided into 4 groups: normal control group,model group,Bushen Jiedu Huoxue group,Yiqi Buxue group.The mice were administrated normal saline,Bushen Jiedu Huoxue Recipe or Yiqi Buxue Recipe once a day continually for 10 days hrough gastric tue.Establish the experimental model of bone marrow suppression at day 8 by ip CTX 100 mg·kg-1,3 days continuously.Peripheral blood cells were counted at day7(the day before the CTX injection)and 1,3,5,7,10 days after the successful model set.;bone merrrow nucleated cell(BMNC)、 colony-forming unit-granulocyte-macraphge(CFU-GM)、 colony-forming unit-erythroyte(CFU-E) and burst-forming unit-erythocyte(BFU-E) were counted 1 day after the successful model set.Result: White blood cell and platelet counts were down to the minimum at day 1 and day 5 after modeling,respectively,and recovered to normal at day 5 and day 10.The number of BMNC,CFU-GM and BFU-E in Yiqi Buxue group and Bushen Jiedu Huoxue group were lower than the normal group,but much higher than that of model group(P0.01).The ratio of Apoptosis of model group was significantly increased(P0.01),which is about 14.7 times of normal control group.The other two groups were decreased obviously than model group(P0.01).GM-CSF was significantly decreased(P0.01) after modeling,however Bushen Jiedu Huoxue Recipe and Yiqi Buxue Recipe could greatly increase it(P0.05).The serum EPO levels didn't show much difference.Conclusion: Preventively use of Bushen Jiedu Huoxue Recipe and Yiqi Buxue Recipe could accelerate the recovery of white blood cell and platelet,and Bushen Jiedu Huoxue Recipe was better on promoting platelet to recovery.Both of Bushen Jiedu Huoxue Recipe and Yiqi Buxue Recipe can reduce the decrease of BMNC,CFU-GM and BFU-E,which was better in Bushen Jiedu Huoxue group;and can less the ratio of apoptosis of bone marrow cells,which was better in Yiqi Buxue group.