Composition of High Molecular Weight Glutenin Subunits in 89 Lines from a Distant Hybridization

The compositions of highmolecularweight glutenin subunits (HMWGS) in 89 lines from a distant hybridization were analyzed using SDSPAGE method in this experiment. The results showed that the HMWGS composition of 89 lines were extremely abundant. 3,6 and 9 different HMWGS types were found in the loci of GluA1, GluB1 and GluD1, respectively. After combining of three loci, there were 30 compositions types. At GluA1 locus, the frequency of subunit 1 (57.31%) was the highest. At GluB1 locus, the frequencies of subunits 7+8 (44.94%) and subunits 7+9 (38.83%) were relatively high. Variations of HMWGS at GluD1 were the most abundant, among which the frequency of subunits 5+10 (38.20%),which were regarded as the highest quality subunits,was the highest. And there were other new allelic genes. Using distant hybridization to improve wheat quality was very useful in China.