Preparation of Soy Peptides by Hydrolyzing Soybean Powder with Combined Enzymes

The effects of substrate concentration,pH,temperature,enzyme dosage,accelerant of enzymatic hydrolysis and time on the hydrolysis of soybean powder by the combined enzymes of Alcalase and papain were studied in this paper.The optimal conditions of enzymatic hydrolysis were obtained by using one-factor and orthogonal design,which were as follows: substrate concentration of 4.5%,combined enzyme dosage(the ratio of Alcalase to papain 2∶1) of 2640U/g,5mmol/L of Mn2+,pH 8.5,60℃,and reaction time of 180min.Under the optimal conditions,the degree of hydrolysis(DH) could reach 17.8%.