Leptin promotes migration and invasion of human glioma U87MG cells

Objective: To investigate the effect of leptin on the migration and invasion of human glioma U87MG cells,and explore its molecule mechanism.Methods: U87MG cells were treated with leptin,the cell migration was analyzed by cell scratch assay,cell invasion was detected by Transwell,and mRNA and protein expressions of MMP-2 and MMP-9 in U87MG cells were observed by RT-PCR and Western blotting assay.Results: Leptin significantly promoted migration([152.42±3.29] vs [83.24±2.61] μm,P0.05),and invasion([31.78±5.04] vs [17.03±2.41]/field,P0.05) of U87MG cells;the mRNA and protein expressions of MMP-2 and MMP-9 were significantly upregulated by leptin in U87MG cells(mRNA: [0.76±0.04] vs [0.35±0.02],[0.84±0.02] vs [0.41±0.06],P0.05;protein: [0.79±0.03] vs [0.23±0.01],[0.81±0.05] vs [0.39±0.03],P0.05);MMP inhibitor GM6001 reversed U87MG cell migration([82.05±2.98] vs [81.76±3.25] μm,P0.05] and invasion([19.23±2.46] vs [18.02±1.98]/field,P0.05) induced by leptin.Conclusion: Leptin can promote the invasion and migration of human glioma U87MG cells,which might related with the upregulation of MMP-2 and MMP-9.