2018•Unpublished venueRequires access
Expression of leptin receptor in gliomas and the effect of exogenous leptin on human glioma U251 cell line
Hongmei Liu, Xianmin Bu, Fangfang Xu, Guoan Zhang, Bin Wu, Haibin Wang, Wen Cui
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Abstract
Objective
To clarify the expression of leptin and leptin receptor in normal brain tissues and gliomas and investigate the effect of exogenous leptin on the proliferation, migration and invasion of human glioma U251 cell line.
Methods
Immunohistochemical staining was used to detect the expression of leptin and leptin receptor in 50 cases of different grades of glioma tissues and 20 cases of normal brain tissues.The effects of exogenous leptin on proliferation, migration and invasion of U251 cells were detected by MTT assay, cell scratch assay and Transwell invasion assay.
Results
(1)The positive expression rates of leptin and leptin receptors in glioma tissues were 50.0% and 92.0%, respectively.(2)Proliferation activity: leptin concentrations of 0 ng/ml, 10 ng/ml, and 50 ng/ml had no significant difference in the proliferation of U251 cells (absorbance: 0.263±0.015, 0.273±0.017 and 0.277±0.006, respectively), and the leptin concentration of 100 ng/ml had a significant effect on the proliferation of U251 cells (absorbance: 0.315±0.005, P<0.05). (3)Migration ability: the migration rate of U251 cells treated with different concentrations of leptin increased significantly with the passage of time, and the migration rate was most significant at the concentration of 100 ng/ml ((93.313±3.080)%), and the difference was statistically significant(P<0.05). (4)Invasive ability: with the increase of leptin concentration and the prolongation of the action time, the invasive ability of U251 cells was enhanced.When leptin was used at a concentration of 100 ng/ml, the number of penetrating cells were the biggest(135±2).
Conclusion
Leptin and leptin receptors are involved in the occurrence of gliomas; and exogenous leptin promotes the proliferation of U251 cells and has time and dose dependability on the migration and invasion of U251 cells.
Key words:
Glioma; Leptin; Leptin receptor; U251 cell; Proliferation; Migration; Invasion