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2004•He'nan nongye kexueRequires access

Studies on the Technique of Tissue Culture and Rapid Propagation of Lily

Wang Yong

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Abstract

Using the edible bulb of Lilum from xinyang as the explant,a method for rapid propagation of Lily was established,based on MS medium. Among 16 medium combinations with NAA,KT and 6-BA concentrations,the appropriate mediums for inducing adventitious buds was MS+1.0 mg/L 6-BA+0.1 mg/L NAA+1.0 mg/L KT;The best optimum medium for differentiation and subculture was MS+1.0 mg/L 6-BA+0.01 mg/L NAA+0.5 mg/L KT.The best medium for rooting was MS+0.1 mg/L IBA.The appropriate calture condition for bud induce and rapid propagation was light period of 16 h and temp of (25±1) ℃.

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What this paper is about

Using the edible bulb of Lilum from xinyang as the explant,a method for rapid propagation of Lily was established,based on MS medium. Among 16 medium combinations with NAA,KT and 6-BA concentrations,the appropriate mediums for inducing adventitious buds was MS+1.0 mg/L 6-BA+0.1 mg/L NAA+1.0 mg/L KT;The best optimum medium for differentiation and subculture was MS+1.0 mg/L 6-BA+0.01 mg/L NAA+0.5 mg/L KT.The best medium for rooting was MS+0.1 mg/L IBA.The appropriate calture condition for bud induce and rapid propagation was light period of 16 h and temp of (25±1) ℃.

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Available abstract

Using the edible bulb of Lilum from xinyang as the explant,a method for rapid propagation of Lily was established,based on MS medium. Among 16 medium combinations with NAA,KT and 6-BA concentrations,the appropriate mediums for inducing adventitious buds was MS+1.0 mg/L 6-BA+0.1 mg/L NAA+1.0 mg/L KT;The best optimum medium for differentiation and subculture was MS+1.0 mg/L 6-BA+0.01 mg/L NAA+0.5 mg/L KT.The best medium for rooting was MS+0.1 mg/L IBA.The appropriate calture condition for bud induce and rapid propagation was light period of 16 h and temp of (25±1) ℃.

Key concepts: Subculture (biology), Explant culture, Tissue culture, Bulb, Botany, Horticulture, Murashige and Skoog medium, Biology

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