2011•Zhongguo yaofangRequires access
Content Determination of Baicalin,Chlorogenic Acid,Forsythin and Forsythoside A in Shuanghuanglian Tablets by HPLC
Yang Qi-chen
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Abstract
OBJECTIVE:To establish a method for simultaneously determination of baicalin,chlorogenic acid,forsythin and forsythoside A in Shuanghuanglian tablets.METHODS:HPLC method was carried out on a Hedera ODS 2 column with mobile phase consisted of methanol-0.2% phosphoric acid solution(gradient elution) at the flow rate of 1.0 mL·min-1.The detection wavelength was 324 nm and 280 nm.Injection volume was 10 μL and column temperature was 30 ℃.RESULTS:The linear ranges of baicalin,chlorogenic acid,forsythin,Forsythosid A were in the range of 28.525～342.3 μg·mL-1(r=0.999 9),4.56～54.72 μg·mL-1(r=0.999 5),1.55～18.6 μg·mL-1(r=0.999 4),1.81～21.72 μg·mL-1(r=0.999 6),respectively.The average recoveries of four active ingredients were 98.12%,96.99%,103.16%,100.14%,respectively(n=6).CONCLUSION:The method is simple,feasible and very suitable for the quality control of Shuanghuanglian tablets.