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2010•Journal of Jiangxi University of Traditional Chinese MedicineRequires access

Simultaneous determination of chlorogenic acid,baicalin and forsythin in Shuang-huang-lian Preparations by reversed phase high performance liquid chromatography

Meitian Xiao

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Abstract

Objective:To establish HPLC method for simultaneous determination of three marker components in different Shuang-huang-lian preparations. Methods:HPLC assay was carried out using a Amethyst C18-P column. The mobile phase was consisted acetonitrile-4% phosphoric acid.The flow rate was 1.0 ml·min-1.The wavelength was set at 230 nm.The column temperature was 30 ℃. Results:The calibration curves were linear over the concentration range of 7.52~150.40 μg·min-1 for chlorogenic acid,4.98~99.60 μg·min-1 for forsythin,and 0.76~15.20 μg·min-1 for baicalin,respective.The correlation co-efficiencies of all curves were better than 0.9988.The average recoveries of three components were 96.75%(RSD=1.28%),99.58%(RSD=1.60%),104.48%(RSD=2.96%),respectively. Conclusion:The developed HPLC method may be used for the quality control of Shuanghuanglian preparations.

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Objective:To establish HPLC method for simultaneous determination of three marker components in different Shuang-huang-lian preparations. Methods:HPLC assay was carried out using a Amethyst C18-P column. The mobile phase was consisted acetonitrile-4% phosphoric acid.The flow rate was 1.0 ml·min-1.The wavelength was set at 230 nm.The column temperature was 30 ℃. Results:The calibration curves were linear over the concentration range of 7.52~150.40 μg·min-1 for chlorogenic acid,4.98~99.60 μg·min-1 for forsythin,and 0.76~15.20 μg·min-1 for baicalin,respective.The correlation co-efficiencies of all curves were better than 0.9988.The average recoveries of three components were 96.75%(RSD=1.28%),99.58%(RSD=1.60%),104.48%(RSD=2.96%),respectively. Conclusion:The developed HPLC method may be used for the quality control of Shuanghuanglian preparations.

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Available abstract

Objective:To establish HPLC method for simultaneous determination of three marker components in different Shuang-huang-lian preparations. Methods:HPLC assay was carried out using a Amethyst C18-P column. The mobile phase was consisted acetonitrile-4% phosphoric acid.The flow rate was 1.0 ml·min-1.The wavelength was set at 230 nm.The column temperature was 30 ℃. Results:The calibration curves were linear over the concentration range of 7.52~150.40 μg·min-1 for chlorogenic acid,4.98~99.60 μg·min-1 for forsythin,and 0.76~15.20 μg·min-1 for baicalin,respective.The correlation co-efficiencies of all curves were better than 0.9988.The average recoveries of three components were 96.75%(RSD=1.28%),99.58%(RSD=1.60%),104.48%(RSD=2.96%),respectively. Conclusion:The developed HPLC method may be used for the quality control of Shuanghuanglian preparations.

Key concepts: Baicalin, Chromatography, Chlorogenic acid, High-performance liquid chromatography, Chemistry, Phosphoric acid, Calibration curve, Detection limit

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Simultaneous determination of chlorogenic acid,baicalin and forsythin in Shuang-huang-lian Preparations by reversed phase high performance liquid chromatography — Research Paper | ScholarLens