ScholarLens Back to search
2009•Journal of Medical ForumRequires access

Growth Inhibitory Effect and Inducing Apoptosis Effect of Oridonin on Human Breast Cancer MCF-7 Cells

LI Xike

Open publisher page 0 citations

Abstract

Objective To investigate the growth inhibitory effect and inducing apoptosis effect of oridonin on MCF-7 cells.Methods Different concentrations of oridonin were added in culture medium.The growth inhibitory rate of the MCF-7 cells was measured by MTT assay,cell apoptotic rate was detected by flow cytometry(FCM),morphology of apoptosis was observed by Hoechst 33258 fluorescence staining.Results Oridonin could inhibit the growth of MCF-7 cells and induce cell apoptosis significantly.The suppression was both in a time-and dose-dependent manner.Marked changes of apoptosis including condensation of chromatin and nuclear fragmentation were observed very clearly by Hoechst 33258 fluorescence staining.Conclusion Oridonin had growth inhibitory effect on MCF-7 cells in vitro,which associated with the cell apoptosis.

About this research paper

What this paper is about

Objective To investigate the growth inhibitory effect and inducing apoptosis effect of oridonin on MCF-7 cells.Methods Different concentrations of oridonin were added in culture medium.The growth inhibitory rate of the MCF-7 cells was measured by MTT assay,cell apoptotic rate was detected by flow cytometry(FCM),morphology of apoptosis was observed by Hoechst 33258 fluorescence staining.Results Oridonin could inhibit the growth of MCF-7 cells and induce cell apoptosis significantly.The suppression was both in a time-and dose-dependent manner.Marked changes of apoptosis including condensation of chromatin and nuclear fragmentation were observed very clearly by Hoechst 33258 fluorescence staining.Conclusion Oridonin had growth inhibitory effect on MCF-7 cells in vitro,which associated with the cell apoptosis.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To investigate the growth inhibitory effect and inducing apoptosis effect of oridonin on MCF-7 cells.Methods Different concentrations of oridonin were added in culture medium.The growth inhibitory rate of the MCF-7 cells was measured by MTT assay,cell apoptotic rate was detected by flow cytometry(FCM),morphology of apoptosis was observed by Hoechst 33258 fluorescence staining.Results Oridonin could inhibit the growth of MCF-7 cells and induce cell apoptosis significantly.The suppression was both in a time-and dose-dependent manner.Marked changes of apoptosis including condensation of chromatin and nuclear fragmentation were observed very clearly by Hoechst 33258 fluorescence staining.Conclusion Oridonin had growth inhibitory effect on MCF-7 cells in vitro,which associated with the cell apoptosis.

Key concepts: Apoptosis, MCF-7, Fragmentation (computing), Flow cytometry, DNA fragmentation, Growth inhibition, Inhibitory postsynaptic potential, Chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Growth Inhibitory Effect and Inducing Apoptosis Effect of Oridonin on Human Breast Cancer MCF-7 Cells — Research Paper | ScholarLens