Effects of Interleukin-10 on Histopathological Changes in Rats with Acute Necrotizing Pancreatitis

Objective To investigate the effects of recombinant human interleukin-10(IL-10) on histopathological changes in rats with acute necrotizing pancreatitis(ANP).Methods Ninety-two male SD rats were randomly divided into three groups:normal control group(Group C,n=24),ANP group(Group A,n=36) and IL-10 interference group(Group I,n=32).Group A received three intraperitoneal injection of 6% L-arginine(3×1.0 mg/g) at hourly intervals.Group I was treated with 10 000 unites of intraperitioneal recombinant human IL-10 at the 2nd,5th and 8th hour after the last injection of L-arginine.Group C received three intraperitoneal injection of 0.9% saline alone at hourly intervals three times.Rats were killed at the 4th,12th,24th and 36th hour after the last L-arginine injection.Histopathological scores were recorded according to the edema,inflammation and necrosis of pancreatic tissues in three groups.Results Pancreatic histopathological scores in Group A were higher than those in Group C at the 4th,12th,24th,36th hour after the last L-arginine injection(P0.01),the highest was at the 36th hour;Pancreatic histopathological scores in Group I were lower than those in Group A at the 12th,24th,36th hour(P0.05);The serum amylase level at each time point in Group I were lower than those in Group A(P0.05).Conclusion Early application of recombinant human IL-10 can attenuate ANP inflammatory response and relieve the histopathological injury,which may be a new therapy for acute necrotizing pancreatitis.