Effect of Qingyitang on IL-6 IL-10 and Intestinal Permeability in Rats with Acute Necrotizing Pancreatitis

Objective: To investigate the effect and mechanism of Qingyitang on inflammation and gut dysfunction in rats with acute necrotizing pancreatitis (ANP). Methods: 72 rats were randomly divided into 3 groups with 24 rats in each group. The rats of two groups were treated by injecting 0.5 ml/100g of 3.8 % sodium taurocholate beneath the pancreatic capsule to get the model of ANP. The 48 rats were divided into the ANP group (A group) and the ANP group treated with Qingyitang (B group). The other 24 rats were sham operation group (S group). The rats of each group were divided into 24h group, 48h group and 72h group. Eternal nutrition was given to rats in each group 12 hours after operation. Rats in group B were given Qingyitang (2.5 ml/100 g ) 2 times every day. Rats in other groups were treated with same volume of normal saline solution. Rats were sacrificed by abdominal aorta exsanguinations at 24th, 48th and 72nd hour after the operation. The blood samples were collected for analysis of serum amylase, IL-6, IL-10 and D-Lactate. Results: The serum IL-10 level of group B was higher than that of group A at 48th hour (P0.05); The serum amylase, IL-6 and D-Lactate levels of group B were lower than that of group A (P0.01), was higher than that of group A at 72nd hour (P0.01). Conclusion: Qingyitang improves the IL-10 level and maintain the intestinal permeability.