Study on the Relation between Duration of Cultivation of Plant and Contents of the Eight Kinds of Ginsenoside in the Panax ginseng by RP-HPLC

Objective:To find the relationship between growing duration of plant and contents of the eight major ginsenoside - Rg1, Re, Rb1, Rf , Re , Rh1, Rb2 and Rd in the Panax ginseng by HPLC, and probe to evaluate the quality of Panax ginseng by the contents and ratios of major ginsenosides. Method: 42 samples of radix ginseng of different ages or specifications were collected , and ginsenoside - Rg1 ,Re,Rb1, Rf .Rc,Rh1 ,Rb2 and Rd have been used as standards to test the samples qualitatively or quantitatively by RP - HPLC method. Test method Ⅰ :The column used was Supelco ODS-C18 column(250 mm×4. 6 mm, 5 μm) ,CH3CN -0. 5% H3PO4(99= 400, pH 2. 6)as mobile phase, at 1.0 mL·?min-1 flow rate, the column temperature was controlled at 35 ℃ ,the detection wavelength was set at 203 nm. Test method Ⅱ:The column used was HIQ Sil - C18 column (250 mm ×4. 6 mm, 5μm) . The mobile phase used were : A was CH3 CN; B was 0. 2% H3 PO4 ( pH 1.9). Time program : A - B (18:82) 0 -65 min 65 -95 min 95-115 min→A-B(46:54)→A-B(87:13)→A-B(18:82)to115-120min,A-B constantly, at 1. 0 mL·min-1 flow rate. The column temperature was controlled at 35℃,the detection wavelength was set at 203 nm. Results: HPLC chromatograms of 42 samples contained almost all ginsenoside compounds information , were analyzed. As well as 6 ginsenosides were determined. Conclusion: There are similar ginsenosides in the samples of Yuanshen,Yishanshen and Shanshen. There isn' t notable difference on the contents of ginsenoside - Rg1 ,Re,Rb1, Rf,Rc and Rh1 within the main roots of different cultivated periods in Yuanshen in comparison with that in Yishan shen and Shanshen. There is considerably diversity in the different parts of drug. Especially in the fibrous root and Lutou. Moreover,the distribution and proportion of the major ginsenosides in various parts of whole radix ginseng differ significantly. These valuable results can be effectively used in evaluation of radix ginseng and ginseng products.