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[Determination of twelve ginsenosides in Panax ginseng by HPLC].

Xiuying Xu, Yimin Zheng, FU Shan-quan, Ying hui Zhao, Jie Li, Linlin Wang

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Abstract

OBJECTIVE: To determine the contents of twelve ginsenosides in the root of Panax ginseng by HPLC. METHOD: The analysis is carried out at room temperature on a Luna NH2 column (4.6 mm x 150 mm, 5 microm) eluted with acetonitrile and water as the mobile phases in a gradient elution. The flow-rate was 1.0 mL x min(-1), the detection wavelength was 203 nm. RESULT: Twelve ginsenosides (Rh2, Rh1, Rg2, Rg3, Rg1, Rf, Re, Rd, Re, Rb2, Rb3, Rb1) were separated at baseline within 60 min with good linearity (r > or = 0.999 5). The recovery rates were 98.1%, 95.3%, 96.1%, 95.6%, 97.3%, 98.6%, 98.0%, 96.4%, 96.1%, 97.6%, 96.8%, 96.9% (RSD < or = 3.0%). CONCLUSION: The method was simple,fast and could control the quality of P. ginseng effectively.

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OBJECTIVE: To determine the contents of twelve ginsenosides in the root of Panax ginseng by HPLC. METHOD: The analysis is carried out at room temperature on a Luna NH2 column (4.6 mm x 150 mm, 5 microm) eluted with acetonitrile and water as the mobile phases in a gradient elution. The flow-rate was 1.0 mL x min(-1), the detection wavelength was 203 nm. RESULT: Twelve ginsenosides (Rh2, Rh1, Rg2, Rg3, Rg1, Rf, Re, Rd, Re, Rb2, Rb3, Rb1) were separated at baseline within 60 min with good linearity (r > or = 0.999 5). The recovery rates were 98.1%, 95.3%, 96.1%, 95.6%, 97.3%, 98.6%, 98.0%, 96.4%, 96.1%, 97.6%, 96.8%, 96.9% (RSD < or = 3.0%). CONCLUSION: The method was simple,fast and could control the quality of P. ginseng effectively.

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Available abstract

OBJECTIVE: To determine the contents of twelve ginsenosides in the root of Panax ginseng by HPLC. METHOD: The analysis is carried out at room temperature on a Luna NH2 column (4.6 mm x 150 mm, 5 microm) eluted with acetonitrile and water as the mobile phases in a gradient elution. The flow-rate was 1.0 mL x min(-1), the detection wavelength was 203 nm. RESULT: Twelve ginsenosides (Rh2, Rh1, Rg2, Rg3, Rg1, Rf, Re, Rd, Re, Rb2, Rb3, Rb1) were separated at baseline within 60 min with good linearity (r > or = 0.999 5). The recovery rates were 98.1%, 95.3%, 96.1%, 95.6%, 97.3%, 98.6%, 98.0%, 96.4%, 96.1%, 97.6%, 96.8%, 96.9% (RSD < or = 3.0%). CONCLUSION: The method was simple,fast and could control the quality of P. ginseng effectively.

Key concepts: Ginseng, Chromatography, Gradient elution, Chemistry, Ginsenoside, Ginsenoside Rg1, High-performance liquid chromatography, Araliaceae

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