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2011•Science and Technology of Food IndustryRequires access

Study on the comparison of different methods for deproteinization from crude polysaccharide of Eucommia ulmoides Oliver

Jian Sun

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Abstract

Sevag method,Trichloroacetic acid method and HCl method were used to remove protein from crude polysaccharide of Eucommia ulmoides Oliver and the percentage of deproteinization,the loss of polysaccharide and the loss of total antioxidant ability ( TAA) were determined. The results showed that with concentration of trichloroacetic acid 3%,deproteinization was effective and TAA didn’t change.In this condition,the percentage of deproteinization was 53.32%,the loss of polysaccharide was 3.67%.

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What this paper is about

Sevag method,Trichloroacetic acid method and HCl method were used to remove protein from crude polysaccharide of Eucommia ulmoides Oliver and the percentage of deproteinization,the loss of polysaccharide and the loss of total antioxidant ability ( TAA) were determined. The results showed that with concentration of trichloroacetic acid 3%,deproteinization was effective and TAA didn’t change.In this condition,the percentage of deproteinization was 53.32%,the loss of polysaccharide was 3.67%.

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Available abstract

Sevag method,Trichloroacetic acid method and HCl method were used to remove protein from crude polysaccharide of Eucommia ulmoides Oliver and the percentage of deproteinization,the loss of polysaccharide and the loss of total antioxidant ability ( TAA) were determined. The results showed that with concentration of trichloroacetic acid 3%,deproteinization was effective and TAA didn’t change.In this condition,the percentage of deproteinization was 53.32%,the loss of polysaccharide was 3.67%.

Key concepts: Eucommia ulmoides, Polysaccharide, Trichloroacetic acid, Chemistry, Chromatography, Biochemistry, Traditional Chinese medicine, Medicine

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