Effect of deproteinization methods on the total antioxidant ability of polysaccharide from Valeriana jatamansi

Objective:To study the effect of three different deproteinization methods on the total antioxidant ability( TAA) of polysaccharide from Valeriana jatamansi.Method: Sevag method,Trichloroacetic acid method and HCl method were used to remove protein from crude polysaccharide of V.jatamansi and the percentage of deproteinization,the loss of polysaccharide and the loss of TAA were determined.Result: The TAA loss of polysaccharide was minimal by Sevag method.The percentage of deproteinization was 41.71% ,the loss of polysaccharide was 46.38% ,and the loss of TAA was 11.62% ,When the polysaccharide solution was treated for 6 times by Sevag method.Conclusion: Sevag method was suitable for the deproteinization of crude polysaccharide from V.jataman-si.