Simultaneous Determination of Notoginsenoside R_1 and Ginsenoside Rg_1 Contents in Shuxiong Tablets by HPLC

Objective:An RP-HPLC method was developed to determine the contents of notoginsenoside R_1 and ginsenoside Rg_1 simultaneously in Shuxiong tablets.Methods:Kromasil ODS(250 mm×4.6 mm,5 μm)was used,the mobile phase was acetonitrile-water-phospharic acid(21.5:78.5:0.02)at a flow rate of 1.0 mL· min~(-1) The UV detection wavelength was 203nm,and the column temperature was room temperature.Results:The linear range was 2.5-49.6 μg·mL~(-1)(r=0.9997,n=7)for notoginsenoside R_1 and 23.6-472.0 μg·mL~(-1)(r =0.9999,n=7)for ginsenoside Rg_1.The average recoveries of notoginsenoside R_1 and ginsenoside Rg_1 were 98.9% (n=9) and 99.1%(n=9)respectively.Conclusion:The method was found to be simple and accurate for simultaneous analysis of notoginsenoside R_1 and ginsenoside Rg_1 in Shuxiong tablets,and provided a reliable way for evaluating the quality of it.