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Cloning of Bovine GC-rich Fcγ2R cDNA by PCR

Songlin Qiao

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Abstract

The cDNA of bovine Fcγ2R with a GC-content 64 percent was cloned by improved PCR using a pair of primers based on previously cloned boFcγ2R.The cloned boFcγ2R cDNA was 795 nucleotides long and deduced 265 amino acids. When it was compared with the published sequence, 3 mutant sites was found. The improved PCR method can be widely available for amplification of a GC-rich template.

About this research paper

What this paper is about

The cDNA of bovine Fcγ2R with a GC-content 64 percent was cloned by improved PCR using a pair of primers based on previously cloned boFcγ2R.The cloned boFcγ2R cDNA was 795 nucleotides long and deduced 265 amino acids. When it was compared with the published sequence, 3 mutant sites was found. The improved PCR method can be widely available for amplification of a GC-rich template.

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Available abstract

The cDNA of bovine Fcγ2R with a GC-content 64 percent was cloned by improved PCR using a pair of primers based on previously cloned boFcγ2R.The cloned boFcγ2R cDNA was 795 nucleotides long and deduced 265 amino acids. When it was compared with the published sequence, 3 mutant sites was found. The improved PCR method can be widely available for amplification of a GC-rich template.

Key concepts: Complementary DNA, Cloning (programming), Molecular biology, Rapid amplification of cDNA ends, Molecular cloning, Biology, Polymerase chain reaction, Mutant

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