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2009•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Content of Quertetin and Kaempferol in Kudindcha Tablets by RP-HPLC

Meng Da-ping

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Abstract

Objective To establish an RP-HPLC method for determination of the content of quertetin and kaempferol in Kudindcha tablets.Methods The Nova-Pak C18 column(3.9 mm×300 mm,4.0μm) was used.The mobile phase was methanol-0.4%H3PO4(50∶50),the detection wavelength was 360nm,the flow rate was 0.7 ml·min-1,the temperature was 40℃.Results The linear range of quertetin was 0.012 99~0.649 6 μg,the linear range of kaempferol was 0.013 34~0.667 2 μg.The average recovery was 97.03%and 97.34%,RSD was 1.16%(n=6) and 1.42%(n=6).Conclusion This method is simple,rapid and accurate.It can be used for determination of the content of quertetin and kaempferol in Kudindcha tablets.

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Objective To establish an RP-HPLC method for determination of the content of quertetin and kaempferol in Kudindcha tablets.Methods The Nova-Pak C18 column(3.9 mm×300 mm,4.0μm) was used.The mobile phase was methanol-0.4%H3PO4(50∶50),the detection wavelength was 360nm,the flow rate was 0.7 ml·min-1,the temperature was 40℃.Results The linear range of quertetin was 0.012 99~0.649 6 μg,the linear range of kaempferol was 0.013 34~0.667 2 μg.The average recovery was 97.03%and 97.34%,RSD was 1.16%(n=6) and 1.42%(n=6).Conclusion This method is simple,rapid and accurate.It can be used for determination of the content of quertetin and kaempferol in Kudindcha tablets.

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Available abstract

Objective To establish an RP-HPLC method for determination of the content of quertetin and kaempferol in Kudindcha tablets.Methods The Nova-Pak C18 column(3.9 mm×300 mm,4.0μm) was used.The mobile phase was methanol-0.4%H3PO4(50∶50),the detection wavelength was 360nm,the flow rate was 0.7 ml·min-1,the temperature was 40℃.Results The linear range of quertetin was 0.012 99~0.649 6 μg,the linear range of kaempferol was 0.013 34~0.667 2 μg.The average recovery was 97.03%and 97.34%,RSD was 1.16%(n=6) and 1.42%(n=6).Conclusion This method is simple,rapid and accurate.It can be used for determination of the content of quertetin and kaempferol in Kudindcha tablets.

Key concepts: Kaempferol, Chromatography, High-performance liquid chromatography, Content determination, Chemistry, Linear range, Quercetin, Detection limit

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