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Content Determination of Kaempferol in Zhemu Syrup by HPLC

Fang Qian, HE Bai-jiang

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Abstract

OBJECTIVE:To establish a method for the content determination of kaempferol in Zhemu syrup.METHODS:HPLC method was adopted.The determination was performed on Calesil ODS-100 C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of methanol-0.1% acetic acid(gradient elution)at a flow rate of 1 mL·min-1.The detection wavelength was set at 360 nm.RESULTS:The linear range of kaempferol was 1～50 μg·mL-1(r=0.999 8).The average recovery rate was 99.77%(RSD=1.21%,n=9).CONCLUSION:The method is rapid,simple and specific,and it can be used for the quality control of Zhemu syrup.

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What this paper is about

OBJECTIVE:To establish a method for the content determination of kaempferol in Zhemu syrup.METHODS:HPLC method was adopted.The determination was performed on Calesil ODS-100 C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of methanol-0.1% acetic acid(gradient elution)at a flow rate of 1 mL·min-1.The detection wavelength was set at 360 nm.RESULTS:The linear range of kaempferol was 1～50 μg·mL-1(r=0.999 8).The average recovery rate was 99.77%(RSD=1.21%,n=9).CONCLUSION:The method is rapid,simple and specific,and it can be used for the quality control of Zhemu syrup.

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Available abstract

OBJECTIVE:To establish a method for the content determination of kaempferol in Zhemu syrup.METHODS:HPLC method was adopted.The determination was performed on Calesil ODS-100 C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of methanol-0.1% acetic acid(gradient elution)at a flow rate of 1 mL·min-1.The detection wavelength was set at 360 nm.RESULTS:The linear range of kaempferol was 1～50 μg·mL-1(r=0.999 8).The average recovery rate was 99.77%(RSD=1.21%,n=9).CONCLUSION:The method is rapid,simple and specific,and it can be used for the quality control of Zhemu syrup.

Key concepts: Chromatography, Kaempferol, Chemistry, Gradient elution, Content determination, High-performance liquid chromatography, Recovery rate, Acetic acid

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