The promotion of Yiguanjian on the recovery of liver fibrosis through mesenchymal stem cells: an experimental study

Objective To investigate the effect of bone marrow mesenchymal stem cells on liver fibrosis reversal,understand the role of Yiguanjian on this process,and interpret the mechanism of Yiguanjian on the recovery of liver fibrosis. Methods we established liver fibrosis rat model. 60 male SD rats were divided into 5 groups: Normal group,model group,western medicine group,mesenchymal stem cells group and Yiguanjian combined with bone marrow mesenchymal stem cells group,There were 12 rats in each group. Except the normal group,hepatic fibrosis model was established. Isolation,culture and labeled bone marrow mesenchymal stem cells. On the third day after CCl4 injection,each rat in mesenchymal stem cells group was given intravenous injection of 5 × 106mmol /L 1 mol bone marrow mesenchymal stem cells. Except equal amounts of bone marrow mesenchymal stem cells,each rat in Yiguanjian combined with bone marrow mesenchymal stem cells group was given Yiguanjian( 2 ml /200 g d) by intragastric administration. Western medicine group was given colchicine( 0. 25 mg /kg d) by intragastric administra-tion. The normal group and model group were fed with same volume of saline daily. Western medicine group,normal group and model group was given intravenous injection of same volume of saline daily. After4 weeks,the serum ALT and AST were tested,the HE staining were used to evaluate the degree of liver inflammation and fibrosis( G)( S),Bone marrow mesenchymal stem cells in liver tissue were observed under fluorescence microscop. Results Compared with the model group and western medicine group,mesenchymal stem cells group and Yiguanjian combined with bone marrow mesenchymal stem cells group had better effect in the improvement of liver function,reduce inflammation,reversing hepatic fibrosis,P 0. 05. Compared with the mesenchymal stem cells group,Yiguanjian combined with bone marrow mesenchymal stem cells group had better effect on improving the liver function,reversing hepatic fibrosis,P 0. 05. Compared with the model group,the area of bone marrow mesenchymal stem cells in liver was greater in mesenchymal stem cells group and Yiguanjian combined with bone marrow mesenchymal stem cells group,P 0. 05. Compared with the other groups,the area of bone marrow mesenchymal stem cells in liver was greater in Yiguanjian combined with bone marrow mesenchymal stem cells group,P 0. 05. Conclusion Bone marrow mesenchymal stem cells can reverse liver fibrosis,Yiguanjian can promote this process,Yiguanjian can promote bone marrow mesenchymal stem cell recruitment to the liver,thus play an effect of recovery of liver fibrosis.