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1994•Acta Biochimica et Biophysica SinicaRequires access

The Preparations, Characterization and Application of AntiHBsAg PreS1(20-47) Polyclonal Antibody

Yang Hai

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Abstract

RBsAg Pre-S1 (21-47) mediates the attachment of HBV to human hepatocytes.A peptide corresponding to the RBsAg pre-S1 (20-47) of subtype adr was synthesized by the conventional solid phase method. Polyclonal antibodies,induced by the peptide-BSA conjugates in rabbit, were purified by affinity chromatography. The affinity-purified polyclonal antibodies, which have high purity, activity and specificity, has been successfully used in detecting natural pre-S1 antigens in the serum of patients and the recombinant fused HBsAg vaccines containing pre-S1 antigen.

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What this paper is about

RBsAg Pre-S1 (21-47) mediates the attachment of HBV to human hepatocytes.A peptide corresponding to the RBsAg pre-S1 (20-47) of subtype adr was synthesized by the conventional solid phase method. Polyclonal antibodies,induced by the peptide-BSA conjugates in rabbit, were purified by affinity chromatography. The affinity-purified polyclonal antibodies, which have high purity, activity and specificity, has been successfully used in detecting natural pre-S1 antigens in the serum of patients and the recombinant fused HBsAg vaccines containing pre-S1 antigen.

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Available abstract

RBsAg Pre-S1 (21-47) mediates the attachment of HBV to human hepatocytes.A peptide corresponding to the RBsAg pre-S1 (20-47) of subtype adr was synthesized by the conventional solid phase method. Polyclonal antibodies,induced by the peptide-BSA conjugates in rabbit, were purified by affinity chromatography. The affinity-purified polyclonal antibodies, which have high purity, activity and specificity, has been successfully used in detecting natural pre-S1 antigens in the serum of patients and the recombinant fused HBsAg vaccines containing pre-S1 antigen.

Key concepts: Polyclonal antibodies, Affinity chromatography, Antibody, Recombinant DNA, HBsAg, Antigen, Chemistry, Peptide

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