Preparation and Identification of the Epitope Peptide P22 from VP2 of Infectious Bursal Disease Virus

The aim of this study was to identify the immunogenicity and the cell binding ability of the linear B cell epitope peptide P22 from VP2 of IBDV.The peptide P22 was synthesized by a solid-phase method.The purified peptide was conjugated with bovine serum albumin(BSA) and cell penetrating peptide(PNT) using SMCC method to prepare the immunogen P22-BSA and the detecting antigen P22-PNT.After immunizing BALB/c mice with P22-BSA,polyclonal antibodies were obtained and identified with ELISA and immunohistochemical assay.Results showed that the titers of the 3 polyclonal antibodies of P22 all reached to 1:105,and the anti-P22 polyclonal antibodies could effectively bind the P22 peptide previously incubated with CEF cells.In this study,the immunogen P22,and the anti-P22 polyclonal antibody were successfully prepared for further study of the infection mechanism of IBDV,and lays the foundation for the design of peptide vaccine and the development of new drugs.