2010•Journal of Nantong UniversityRequires access

Preparation and characterization of polyclonal antibody against human glypican3 N-terminal protein

Zhao Minxing

Abstract

Objective:To prepare rabbit anti-human Glypican3 N-terminal protein polyclonal antibody.Methods:The Glypican3 N-terminal protein was expressed in E.coli Rosetta.The rabbits were immunized by purified protein to produce polyclonal antibody.The anti-sera were identified with ELISA and Western blot analysis.Results:The polyclonal antibody against Glypican3 N-terminal protein was successfully prepared and the titer of antisera was 1:64 000.Conclusion:The polyclonal antibody against human Glypican3 N-terminal protein was successfully prepared.

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Key concepts: Polyclonal antibodies, Antiserum, Antibody, Western blot, Molecular biology, Titer, Chemistry, Biology