The relationship between the changes of immune function and the bacterial translocation and rhubarb intervention in patients with severe acute pancreatitis

Objective: To investigate the relationship between the changes of immune function and the bacterial translocation and rhubarb intervention in patients with severe acute pancreatitis.Methods: Thirty Wistar rats were randomly divided into three groups: Models group of SAP(M,n=10) were induced by injecting adversely 5% sodium taurocholate(0.2 ml·min-1,0.1 ml·100 g-1)into the common biliary-pancreatic duct and saline was used in the rats of Sham operation group(SO,n=10) instead of sodium taurocholate.Therapeutic group(T,n=10) were made as same as M group besides Rhubarb(50mg·kg-1) were injected into their stomach on the instant after operation;Peripheral blood samples were drawn after 24hours of induced SAP,and the percentages of CD4+ and CD8+ lymphocyte were determined by the flow cytometry.Bacterial culture in pancreas,blood and mesenteric lymph nodes(MLN) were made.Results: The percentages of CD4+ and CD4+/CD8+ratio in M group were significantly lower than those in SO group(P0.01).The volume of ascite in M group was much higher than that in SO group(P0.01) and T group(P0.01).However the bacterial positive rates of blood,pancreas and MLN were much higher than that in T group.Conclusion:Damage to cellular immunity function occurs and may be a main cause that results in the bacterial translocation in the early stage of SAP.Rhubarb has therapeutic efficacy on SAP by the improvement of cellular immune function.