Establishment and Identification of siRNA Eukaryotic Expression Vectors of Gene MMP-26

Objective To establish the recombinant plasmid of small interfering RNA(siRNA) against matrix metalloproteinase-26(MMP-26),observe dumbness effect of the MMP-26 expression in human carcinoma,and explore the new method of gene therapy.Methods Recombinant were designed and established by targeting gene MMP-26 and plamid pSUPERIOR.puro based on MMP-26 mRNA sequence.Three pairs of oligonucleotides were synthesized and inserted into plamid pSUPERIOR.puro to generate siRNA eukaryotic expression vectors.DH5α strains were transformed,plasmid were extracted,and the recombinant sequences were identified.Interfering plasmid from GFP and β-actin were established as negative and positive controls for MMP-26.Results The result of recombinant sequence was the same as aim sequence.The recombinant vectors were established successfully.Conclusion siRNA recombinant can be established successfully by RNA interference (RNAi) technique.