Construction of plasmid expression vector of RNA interference targeting MMP-2 gene

Objective To construct the plasmid expression vector of RNA interference(RNAi) targeting matrix metalloproteinase-2(MMP-2) gene for studying the significance of MMP-2 inhibition in against the invasiveness and metastasis of pancreatic cancer.Methods Genome sequences of MMP-2 gene was retrieved from Genbank and cDNA was designed coding expression of s small hairpin RNA(shRNA) for MMP-2 gene in vivo.The cDNA was synthesized and inserted into plasmid pGCsi-U6/Neo/GFP,which was the plasmid expression vector controlled by human U6 promoter.Results The recombinant plasmids pGCsi-MMP-2 containing specific sequences were confirmed by PCR and sequencing.Conclusion The plasmid expression vector of RNAi targeting MMP-2 gene has been successfully constructed.