2003•Journal of Sichuan UniversityRequires access
Molecular Cloning and Sequence Analysis of Orychophragmus violaceus Toc 33
Liao Wen
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Abstract
By using PCR with primers designed according to the Toc33 sequence of Arabidopsis thaliana, two homologous DNA sequences were directly amplified from the genomic DNA of Orychophragmus violaceus. The two PCR fragments were cloned and confirmed by PCR and restriction enzyme digestion. Sequence analysis showed that the two sequences were1475bp, 1573bp and shared 88% identity with each other, their coding region and deduced amino acid sequences also showed very high identity with each other and with Arabidopsis thaliana Toc33 sequence, the result showed that the sequences are highly conserved.