Molecular cloning and sequence analysis of Toc33 gene in Brassica napus

By using primers according to the Toc33 sequence of Arabidopsis thaliana, two homologous DNA sequences were amplified from the genomic DNA of Brassica napus. Sequence analysis showed that the two sequences were 1 370 bp and 1 490 bp respectively; they shared 78% identity with each other, their coding region and deduced amino acid sequences also showed very high identity with Arabidopsis thaliana Toc33 sequence. Based the alignment of the amino acids of Toc33 and Toc34 genes, the evolutionary tree of Brassica napus, Zea mays, Pisum sativum, Arabidopsis thaliana and Orychophragmus violaceus was constructed.