Intercomparison of Determination Methods of 6 Trace Elements in Pollen

[Objective]One digestion can simultaneously determine various trace metal elements to improve the efficiency and to meet the inspection and quarantine system's requirements of rapid,high-efficiency and accuracy.[Methods] Through the test and analysis to find the best method of digestion and determination of 6 trace metal elements of Fe、Cu、Zn、Mn、Pb、Cd,the comparison of atomic absorption spectroscopy(AAS)-wet digestion and Inductively coupled plasma-atomic emission spectrometry(ICP-AES)-microwave digestion method.[Results] Under selected experimental conditions,recovery rate and coefficient of variation of ICP-AES-microwave digestion method was 97.2%～104.1% and 1.19%～3.54% respectively;that of AAS-wet digestion was 95.5%～ 98.7%,and 1.26%～4.58% respectively.Microwave digestion,digestion fast,used less reagent;wet digestion,with a long time digestion,was vulnerable to be polluted with the loss of elements.[Conclusion] ICP-AES method with low detection limit,high precision,linear range,can be used for high,medium and low content in a variety of elements measurements simultaneously.AAS method with low detection limit,small sample volume,cumbersome operation and long time digestion,can only determine 1 element for each time.ICP-AES microwave method,which is simple,rapid and accurate,is suitable for the determination of 6 trace metals of Fe,Cu,Zn,Mn,Cd,Pb in pollen.