Optimization of microwave digestion conditions for 13 elements in marine organisms by ICP-AES

Herein, thirteen elements including Se, Fe, Mn, Zn, Ca, Mg, Sr, As, Cd, Cr, Cu, Pb and Ni in eight kinds of marine organisms were determined by using microwave digestion technique, and applying Inductively Coupled Plasma-Atomic Emission Spectroscopy(ICP-AES). The conditions of microwave digestion were optimized through orthogonal experiment design. The optimum conditions were as follows: the volume ratio of HNO3-H2O2︰6︰1, digestion temperature: 170 ℃, and digestion time: 20 min. The correlation coefficients of linear regression equation for all elements were above 0.999. The recovery rate of the method was in the range of 92.0%~104.8%, and the relative standard deviations were between 0.30% and 2.55%. The detection limit of the method ranged from 0.0010 μg/g to 0.0468 μg/g. The results indicated that the optimized method was convent, sensitivity, accurate and precision, and also can be used for simultaneous multi-element detection. It was suitable for determination of these elements in marine organisms, and also provided scientific foundation for evaluation of safety of seafood.