Sequencing and phylogenetic analysis of the partial 16S rRNA in a Bacillus strain

A Bacillus strain BS501 which had strong antagonistic activity of M.grisea was isolated from the soil of rice field near the Yellow river in Henan.BS501 was determined to be a Bacillus bacteria through morphological observation,physiological and biochemical characterization and electronic microscopy.To further determine the taxonomic status of the strain,the 16S rDNA of BS501genome was amplified,and the sequencing result was submitted to the GenBank database(GenBank accession number is FJ755787).The homology of 16S rDNA sequence of BS501 and Bacillus subtilis,Bacillus licheniformis,other Bacillus spp,and E.coli was compared using BLAST software.The phylogenetic tree showed that BS501 and Bacillus spp bacteria form a large branch in the evolution of relations,and BS501 was the closest to Bacillus subtilis,and was the fartherest to E.coli.Homologous analysis showed that the sequence homology of BS501 and Bacillus subtilis PRE23 was 99%.The result confirmed that the taxonomic status of strain BS501 is Bacillus subtilis.