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2005•Journal of Jilin University ofRequires access

Changes of IGF-I expression in focal cerebral ischemical reperfusion injury model of rats

Zhongxin Xu

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Abstract

Objective To study the expression of insulin-like growth factor-Ⅰ (IGF-Ⅰ) in focal cerebral ischemical reperfusion injury model and its contribution. Methods The focal cerebral ischemia rat models were induced using the filament method, the expressions of IGF-Ⅰ protein and IGF-Ⅰ mRNA was dectected by the method of immunohistochemical staining and in situ hybridization technique. Results The expression of IGF-Ⅰ was very low in normal tissues, while increased mainly in penumbra after cerebral ischemia. The expressions of IGF-Ⅰ protein and IGF-Ⅰ mRNA were 169.64±6.97 and 168.64±6.28,respectively, at 24 h after reperfusion and peaked to 168.86±5.39 and 170.86±5.07 at 48 h after reperfusion. Conclusion Enhanced expression of endogenous IGF-Ⅰ has protective effects on focal cerebral ischemia reperfusion injury.

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Objective To study the expression of insulin-like growth factor-Ⅰ (IGF-Ⅰ) in focal cerebral ischemical reperfusion injury model and its contribution. Methods The focal cerebral ischemia rat models were induced using the filament method, the expressions of IGF-Ⅰ protein and IGF-Ⅰ mRNA was dectected by the method of immunohistochemical staining and in situ hybridization technique. Results The expression of IGF-Ⅰ was very low in normal tissues, while increased mainly in penumbra after cerebral ischemia. The expressions of IGF-Ⅰ protein and IGF-Ⅰ mRNA were 169.64±6.97 and 168.64±6.28,respectively, at 24 h after reperfusion and peaked to 168.86±5.39 and 170.86±5.07 at 48 h after reperfusion. Conclusion Enhanced expression of endogenous IGF-Ⅰ has protective effects on focal cerebral ischemia reperfusion injury.

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Available abstract

Objective To study the expression of insulin-like growth factor-Ⅰ (IGF-Ⅰ) in focal cerebral ischemical reperfusion injury model and its contribution. Methods The focal cerebral ischemia rat models were induced using the filament method, the expressions of IGF-Ⅰ protein and IGF-Ⅰ mRNA was dectected by the method of immunohistochemical staining and in situ hybridization technique. Results The expression of IGF-Ⅰ was very low in normal tissues, while increased mainly in penumbra after cerebral ischemia. The expressions of IGF-Ⅰ protein and IGF-Ⅰ mRNA were 169.64±6.97 and 168.64±6.28,respectively, at 24 h after reperfusion and peaked to 168.86±5.39 and 170.86±5.07 at 48 h after reperfusion. Conclusion Enhanced expression of endogenous IGF-Ⅰ has protective effects on focal cerebral ischemia reperfusion injury.

Key concepts: Penumbra, Reperfusion injury, Immunohistochemistry, Ischemia, Medicine, In situ hybridization, Endogeny, Messenger RNA

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