2010•Journal of Apoplexy and Nervous DiseasesRequires access
The expression development of ATF4 mRNA and protein following focal cerebral ischemia/reperfusion in rats
Liming Tan
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Abstract
Objective To detect the expression development of activating transcription factor 4(ATF4)mRNA and protein after cerebral ischemia/reperfusion in rats.Methods Transient focal cerebral ischemia was induced by middle cerebral artery occlusion(MCAO)for 2 hours followed by reperfusion in spraue-dawley rats.Then the expression of ATF4 protein and/or mRNA were measured with methods of immunohistochemistry and reverse transcription-polymerase chain reaction(RT-PCR)at 1h,3h,6h,12h and 24h after reperfusion in the penumbra of rat cortex.Results In cerebral ischemia model group,the expressions of ATF4 mRNA and protein were up-regulated at 1h after reperfusion.In addition,the expression levels of ATF4 mRNA and protein reached a peak at 6h and 24h after reperfusion,respectively.Conclusion The mRNA and protein levels of ATF4 increase in the penumbra of rat cortex after transient focal cerebral ischemia which suggests that ATF4 may play an important role in the cerebral ischemia and reperfusion injury.