Optimization of Multi-Enzyme Hydrolysis Extraction of Polysaccharide from Garlic under the Effect of Ultrasonic Wave

Take garlic as material to extract polysaccharide.The effects of different extraction methods including hot water extraction,multi-enzymatic method,ultrasonic wave method,ultrasonic-assisted multi-enzymatic hydrolysis,etc.and different enzymes on the extraction yield of garlic polysaccharides are explored in this study.The best method for extraction of garlic polysaccharides is simultaneous treatment of ultrasonic wave and multi-enzyme(pectinase,papain and cellulase).Use orthogonal experiment to optimize the ratio of complex enzyme.The best dosages of pectinase,papain and cellulase are 22.5,160,96U/g respectively.Under these conditions,the extraction yield of garlic polysaccharides is 25.798%.Compared with the traditional hot water extraction method,it has the advantages of higher extraction yield and shorter extraction time.