Study on micro-assisted extracting and antioxidant activity of flavonoids from the persimmon fruit

The optimum process conditions of extracting flavonoids from the persimmon fruit by microwave aided method were studied through factor analysis method and orthogonal experiment,and the antioxidant activity was determined.Results showed that the optimum extraction parameters were identified as follows:ethanol concentration of 70%,microwave power of 600W,microwave time of 150s,material-to-liquid ratio of 1:30,and the extraction amount of flavonoids was 42.4mg/g.The total antioxidant ability of persimmon flavonoids became greater with the concentration range increasing,but inferior to VC.The persimmon flavonoids had certain scavenging action to the hydroxyl free radical,the clearance rate reached 68.70% when the sample density was 0.6mg/mL.This method was simple,repeatable and convenience,and could be used for the extraction and determination of total flavonoids from persimmon.