Research on the Extraction Technology of Total Flavonoids from Persimmon Leaf with the Optimal Enzymatic Method of Response Surface

[Objective] The reference for the extraction of total flavonoids from persimmon leaf was provided through the research on its extraction condition and method.[Method] The enzymatic method was adopted in the extraction of total flavonoids from persimmon leaf.The response surface method with 3 factors at 3 levels,which was based on single factor test,was designed to analyze the all of affecting factors,according to the principle of Box-Benhnken experiment.[Results] The extraction rate of total flavonoids from persimmon leaf being taken as the value for the response surface method and contour,the optimum extraction condition was confirmed as follows:the concentration of the cellulose added,9 mg;the time enzymatic hydrolysis,130 minutes;the temperature,52 ℃;and the pH,4.8,under which condition,the extraction rate was 2.47%.[Conclusion] The method was better with high extraction rate of total flavonoids from persimmon leaf,which could provide the reference for its future industrial production.