Selection and Mutation Breeding of Alkaline Protease-producing Strains

In order to obtain stable high yield of alkaline protease strains,producing alkaline protease strain was treated by tablet mutagenesis method for mutagenic treatment. The results showed that by using milk tablet and Folin measuring enzyme activities,alkaline protease strains were produced from chicken dung upon sample. The strain by nitrosoguanidine( the NTG mutagenesis) and use of rifampicin resistant strains screened two variants,whose protease activity was 34 times and 65 times of the starting strain. Consequently,mutagen nitrosoguanidine could produce high yield of alkaline protease strains.